MSC are present at the ratio of one to 10,000 〜　100,000 in bone marrow　cells. MSC also decreases in number, as we get older. For example, the number of MSC becomes extremely reduced when we reach the age at which we often begin to suffer from periodontal diseases. In the past, therefore, we had to collect as much bone marrow fluid as possible from a patient in order to take enough amount of stem cells necessary for replanting.
Also, the previous cultivation method using human blood serum achieved just ten-fold increase in the number of MSC and so it was very difficult to secure the necessary amount of MSC for medical treatment. We, Two Cells, have made a great success in taking MSC from the marrow of iliac bone and alveolar bones (MSC can be collected from alveolar bone marrow at the time of tooth extraction) and in “super- proliferating” MSＣ over 10,000 times in number. Through our successful method, we are now able to secure enough amount of MSC for replanting purpose just by taking small amount of bone marrow. Our method has successfully reduced the burden of patients. This method is called “MSC Super-Proliferation” utilizing FGF2 (fibroblast growth factor2). This newly-developed method enables us to achieve over-10,000-fold increase in number of MSC, or to proliferate 1,000 times more MSC than the traditional method.
(as shown in Fig.2)

MSC retain the ability to differentiate into various　types of adult cells such as bone cells, cartilage cells, cardiac muscle cells, fat cells, and nerve cells. That is why we pay much attention to MSC for medical purposes and the benefits of patients. MSC can be collected from the bone marrow of every person. For this excellent property, a great number of patients can be relieved from the suffering caused by diseases, if MSC is replanted in them. Thus, many diseases can be cured. MSC is researched and studied all over the world, and the medical potentiality and very promssing. We are going to work on to treat a great number of patients who suffer from the diseases listed in Fig.1 .
In fact, we, Two Cells, together with Dentistry Department, Hiroshima University, have already been committed to utilize MSC in regeneration of periodontal tissues　for treatment for periodontitis.